Earlier this month, GenoMed4All partners and collaborators met to discuss opportunities in OxygenScan protocol harmonization. The Oxygenscan test is particularly relevant for our use case group working on Sickle Cell Disease (SCD), as it measures the relative oxygen pressure at the precise moment red blood cells reach the Point of Sickling (PoS) and thus, describes the defined loss of deformability of red blood cells affected by SCD.

This first meeting was co-chaired by Mar Mañú and Amira Idrizovic from Vall d’Hebron Institute of Research and Minke Rab and Richard van Wijk from UMC Utrecht, and gathered experts from Università  degli Studi di Padova, APHP and additional interested parties from the Hematology department (Laboratory LHUB-ULB) at Erasme Hospital, Brussels.

The focus was on the standardization of key points in the OxygenScan analysis process within GenoMed4All’s practice. Among the critical aspects that need to be considered are: hardware and software settings and machine set-up (e.g. fixed gain and oxygenation for the experiments), blood volume used for the test on red blood cell count, storage temperature for these blood samples, considerations of osmolarity for PVP vials, the pO2 spot…. Participants also agreed on a common methodology to check reproducibility of all experiments.

This is a meaningful first step in setting a baseline for the overall standardization of Oxygenscan tests, which will ultimately shape a compendium of best practices to share with future associate members of GenoMed4All within ERN-EuroBloodNet‘s member network.



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